ABSTRACT
Objective: Extracorporeal Shockwave Lithotripsy [ESWL] is a non-invasive method that is effective at crushing stones in the upper urinary tract. Disturbance of the thiol/disulfide homeostasis, in favor of the disulfide, has been shown to be involved in the disease pathogenesis
Methods: A total of 36 individuals that underwent ESWL had blood samples collected before ESWL [0hrs], 6hrs, and one week after the ESWL. Sera native and total as wells as disulfide amount was measured using an automated method sodium borohydrate [NaBH4] reduction. In addition, Ischemia Modified Albumin [IMA] levels were measured using colorimetric assay method
Results: Native thiol level was reduced at the 6th hour following ESWL compared to baseline. While the ratios of disulfide level, Disulfide/Total Thiol [DTT], Disulfide/Native Thiol [DNT] and IMA level were increased at the 6th hour following ESWL compared to baseline, they were found to be similar with their baseline values at the end of 1st week. Total thiol and native /total thiol did not show any significant change
Conclusion: ESWL treatment disrupts thiol/disulfide homeostasis and the structure of albumin at the acute term. Therefore, it increases protein oxidation and leads to increased oxidative stress. However, this state is transient and returns to normal within the proceeding days
ABSTRACT
Objective: To investigate the effect of retrograde intra-renal surgery [RIRS] on kidneys using the myoinositol oxygenase [MIOX] enzyme. MIOX is a renal tubular-specific novel marker for the early diagnosis of acute kidney injury
Methods: A total of twenty seven individuals that had undergone RIRS to treat kidney stones were included in the study. Biochemical tests were performed on serum samples collected immediately before RIRS [hour 0] and at the 6th and 24th hours after the surgery
Results: The creatinine value at hour 6 was lower than the baseline [hour 0] value [p = 0.0305]. Cystatin C at hour 6 was lower than the value measured at hour 24 [p = 0.0142]. Similarly, MIOX was lower at hour 6 compared to hour 24 [p = 0.0214]. MIOX/creatinine at hour 6 was lower than the value calculated at hour 24 [p = 0.0348]. The basal values of MIOX and creatinine were found to have a positive correlation [correlation coefficient r = 0.5946, p = 0.0035]
Conclusions: Similar to the serum creatinine, the serum MIOX level provides information about kidney functions. RIRS was confirmed to be a safe procedure for the treatment of acute kidney injury with no negative effects on the kidneys